5 培养方法
5.1 选择什么样的培养基？
5.1.1 应该使用基本培养基还是富营养培养基？
5.1.2 都有哪些常用的碳源类型？
5.2 培养条件如何设置？
5.2.1 静置还是振荡培养？
5.2.2 培养温度如何设置？
5.2.3 培养多长时间？
5.3 有哪些常用的培养策略？
5.3.1 什么是连续梯度稀释培养？
5.4 FBA 分析中的培养基
Table S1: Metabolite concentrations used to simulate minimal medium in COMETS. Lactose (lcts) was replaced with alternative carbon sources for individual E. coli colony simulations (glc = 3.5e-6, lac-L = 7.1e-6, or ac = 1.1e-5). Oxygen was lowered to maintain the same concentration with smaller pixel size (i.e. if box length (L)= 0.2 mm then o2 = 1e-5). For the 3-species consortium methylamine was added (mea = 2.0e-6).
| Compound | mM per box |
|---|---|
| ca2[e] | 1000.0 |
| cl[e] | 1000.0 |
| cobalt2[e] | 1000.0 |
| cu2[e] | 1000.0 |
| fe2[e] | 1000.0 |
| fe3[e] | 1000.0 |
| k[e] | 1000.0 |
| lcts[e] | 1.2e-5 |
| mg2[e] | 1000.0 |
| mn2[e] | 1000.0 |
| mobd[e] | 1000.0 |
| nh4[e] | 1000.0 |
| ni2[e] | 1000.0 |
| o2[e] | 6.2e-5 |
| pi[e] | 1000.0 |
| so4[e] | 1000.0 |
| zn2[e] | 1000.0 |
Table S2: Metal Mix used in Hypho minimal media for lab experiments
| Metal | 1000x concentration (mM) |
|---|---|
| \(\ce{ZnSO4}\) | 0.60 |
| \(\ce{CaCl2}\) | 9.98 |
| \(\ce{MnCl2}\) | 0.51 |
| \(\ce{(NH4)6Mo7O24}\) | 1.00 |
| \(\ce{CuSO4}\) | 0.50 |
| \(\ce{CoCl2}\) | 1.00 |
| \(\ce{Na2WO4}\) | 0.17 |
| \(\ce{FeSO4}\) | 8.88 |